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Allplex™ GI-Bacteria(II) Assay

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The Allplex™ GI-Bacteria(II) Assay is a multiplex real-time PCR test that detects hypervirulent Clostridium difficile and E. Coil in gastrointestinal infections through genotyping. Key Benefits Comprehensive testing: simultaneous detection and monitoring of 6 bacteria that cause...

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The Allplex™ GI-Bacteria(II) Assay is a multiplex real-time PCR test that detects hypervirulent Clostridium difficile and E. Coil in gastrointestinal infections through genotyping.

Key Benefits

  1. Comprehensive testing: simultaneous detection and monitoring of 6 bacteria that cause gastrointestinal infections in a single reaction
  2. Multiplexing: individual Ct values for multiple analytes in a single channel of the real-time PCR instrument (MuDT™ technology)
  3. High sensitivity and specificity thanks to the use of DPO™ and TOCE™ technologies
  4. Use of the UDG system (a mixture containing uracil-DNA glycosylase) to prevent autocontamination during the preparation of PCR mixtures
  5. The test system complies with the requirements of the European Regulation on in vitro diagnostics, demonstrating compliance with European standards for safety, public health, and environmental protection (CE IVD)
  6. Convenient workflow: automated data interpretation using the Seegene Viewer software module
  7. Ability to combine this test with other Allplex™ assays for gastrointestinal pathogens

Targets

  • EAEC (enteraggregative E. coli) (aggR)
  • EPEC (enteropathogenic E. coli) (eaeA)
  • Escherichia coli O157 (E. coli O157)
  • ETEC (enterotoxigenic E. coli) (lt/st)
  • Hypervirulent Clostridium difficile (CD hyper)
  • STEC (Shiga toxin-producing E. coli) (stx1/2)
  • Internal Control (IC)
Sample Results Presentation The result indicates the detection of the stx1/stx2 and eaeA genes in the FAM channel with Ct values of 29.91 (Graph 1) and 39.43 (Graph 2), respectively. *Note: For use with the CFX96™ real-time PCR system (Bio-Rad)

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