Control of endogenous and internal extraction
The validation of nucleic acid extraction processes and endogenous controls is crucial in molecular diagnostics, especially when using highly sensitive methods such as real-time PCR. To support this critical step, NZYtech has developed and offers kits for internal nucleic acid ex...
Turnkey supply, installation and training · Service across Ukraine
The validation of nucleic acid extraction processes and endogenous controls is crucial in molecular diagnostics, especially when using highly sensitive methods such as real-time PCR.
To support this critical step, NZYtech has developed and offers kits for internal nucleic acid extraction control, either as an extraction control —by adding it to the lysis buffer prior to sample extraction—or as an amplification control—by introducing it directly into the real-time PCR reaction. To ensure sufficient sample quantity, we recommend using appropriate endogenous controls, depending on the species being studied.
NZYtech kits are lyophilized and compatible with all standard real-time thermocyclers:
| Purpose | NZYtech Kit | Purpose |
| Internal DNA Extraction Control | DNA Internal Extraction Control, 100 reactions, MD08081 | Designed to detect exogenous DNA templates (DNA IEC) in vitro |
| RNA Internal Extraction Control | RNA Internal Extraction Control, 100 reactions, MD08091 | Intended for the detection of exogenous RNA templates (RNA IEC) in vitro |
| Detection of genetic markers in cats, dogs, and humans | Endogenous Control A Real-time PCR Kit, 48 reactions, MD05781 | Intended for the detection of the beta (β)-actin (actB) gene in vitro in several mammals, including humans, cats, and dogs |
| Detection of genetic markers in horses and pigs | Endogenous Control B Real-time PCR Kit, 48 reactions, MD05791 | Intended for the in vitro detection of the beta (β)-actin (actB) gene primarily in mammals, including horses and pigs |
| Detection of genetic markers in fish | Endogenous Control C Real-time PCR Kit, 48 reactions, MD05801 | Intended for the in vitro detection of the 16S rRNA mitochondrial gene in fish |
| Detection of genetic markers in ruminants | Endogenous Control D Real-time PCR Kit, 48 reactions, MD05811 | Intended for the in vitro detection of the beta (β)-actin (actB) gene in several mammals, including ruminants such as cattle and sheep. |
| Detection of genetic markers in birds | Endogenous Control E Real-time PCR Kit, 48 reactions, MD05821 | Intended for the in vitro detection of the beta (β)-actin (actB) gene, primarily in birds |
| Detection of genetic markers in cereals | Endogenous Control F Real-time PCR Kit, 48 reactions, MD05831 | Intended for the in vitro detection of the actin gene in cereal crops, specifically T. aestivum (bread wheat), T. urartu (wild red einkorn wheat), T. dicoccoides (wild emmer), H. vulgare (barley), and C. nutans (common spikelet) |
Technical specifications
- Manufacturer
- NZYtech
- Directions
- Molecular genetic diagnostics
- Methods
- Real-time PCR
- Industries
- Veterinary diagnostics
- Type
- Reagents
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Purchase, delivery and warranty terms
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Click “Price request” and leave your contacts. A manager will clarify the configuration, prepare a commercial offer and answer technical questions within 24 hours.
Delivery and supply
Direct supply from the manufacturer, no intermediaries. Lead time depends on the configuration and is fixed in the offer; we deliver across Ukraine.
Warranty and service
Manufacturer's warranty on the equipment. Installation, qualification and staff training; a service engineer responds within 48 hours.
Procurement documents
Commercial offer with and without VAT, contract specification. CE, ISO, IVD certificates, declarations of conformity and user manuals.
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